Buchi PrepChrom C-700 Uživatelský manuál Strana 1

PrepChrom C-700Operation Manual11593559en

1|General BÜCHILabortechnikAG10/110 PrepChromC700Description QtyInjectionloop,2mL 1Precolumnholdercomplete(incl.stainlesssteelconnecto

9|Takingoutofoperation BÜCHILabortechnikAG100/110 PrepChromC700

BÜCHILabortechnikAG Spareparts|10PrepChromC700 101/11010 Spareparts10.1 GeneralinformationAlwaysstatethepartnumberandproductdescriptio

10|Spareparts BÜCHILabortechnikAG102/110 PrepChromC700Description Qty OrdernumberColumnholderblockingassembly 1 11061489Columnholderconne

BÜCHILabortechnikAG Spareparts|10PrepChromC700 103/110Description Qty OrdernumberModulefortheacquisitionofanexternaldatachannelAnalogic

10|Spareparts BÜCHILabortechnikAG104/110 PrepChromC700

BÜCHILabortechnikAG Appendix|11PrepChromC700 105/11011 Appendix11.1 SolventcharacteristicsfornormalphasecolumnsSolvent SolventstrengthSDip

11|Appendix BÜCHILabortechnikAG106/110 PrepChromC700

BÜCHILabortechnikAG IndexPrepChromC700 107/110IndexNumerical4waysuctionvalve 216wayelectricvalve 21AAGO 8Assemblyorder 39CCheckvalve 

Index BÜCHILabortechnikAG108/110 PrepChromC700

BÜCHILabortechnikAG Safety|2PrepChromC700 11/1102 SafetyThesafetyoftheuserandpersonnelcanonlybeensuredifthesafetyinstructionsand

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2|Safety BÜCHILabortechnikAG12/110 PrepChromC700Fig.2:Exampleofasafetywarning1 Safetysymbol 2 Sourceofdanger3 Instructiontoavoiddang

BÜCHILabortechnikAG Safety|2PrepChromC700 13/1102.4 Generalsafetyrules2.4.1 ResponsibilityoftheoperatorTheheadofthelaboratoryisrespon

2|Safety BÜCHILabortechnikAG14/110 PrepChromC700Additionalwarningmessagescanbefoundwheneveractionsandsituationsdescribedinthismanu

BÜCHILabortechnikAG Technicaldata|3PrepChromC700 15/1103 Technicaldata3.1 Devicedimensions3.2 Technicaldata3.2.1 GeneralDescription ValueWei

3|Technicaldata BÜCHILabortechnikAG16/110 PrepChromC700Description ValueMaximumflowrate 500mL/minTimeconstant 1sec.3.2.3 ValvemoduleDescr

BÜCHILabortechnikAG Technicaldata|3PrepChromC700 17/1103.2.8 EnvironmentalconditionsDescription SpecificationTemperature 5–40°CAltitudeabove

3|Technicaldata BÜCHILabortechnikAG18/110 PrepChromC700

BÜCHILabortechnikAG Descriptionoffunction|4PrepChromC700 19/1104 Descriptionoffunction4.1 PurposeThePrepChromC700isamoderndevicedesi

ImprintProductIdentification:OperationManual,PrepChromC70011593559enPublicationdate:09.2014,VersionB

4|Descriptionoffunction BÜCHILabortechnikAG20/110 PrepChromC7004.2 Deviceoverview4.2.1 FrontandrightsideFig.3:Frontandrightsideview1

BÜCHILabortechnikAG Descriptionoffunction|4PrepChromC700 21/1104.2.2 LeftsideFig.4:Leftsideview1 Columnholder,left 2 Injectionadapter3

4|Descriptionoffunction BÜCHILabortechnikAG22/110 PrepChromC7004.2.3 RearviewFig.5:Rearview1 Mainswitch(5pcs) 2 COMportRS2323 Control

BÜCHILabortechnikAG Descriptionoffunction|4PrepChromC700 23/1104.3 FunctionFig.6:Principleofthechromatographysystem1 6wayelectricalva

4|Descriptionoffunction BÜCHILabortechnikAG24/110 PrepChromC7004.3.3 ControlunitThesystemiscontrolledbythecentralprocessingunitviaa

BÜCHILabortechnikAG Descriptionoffunction|4PrepChromC700 25/1104wayaspirationvalveThe4wayaspirationvalveallowstoperformquaternaryg

4|Descriptionoffunction BÜCHILabortechnikAG26/110 PrepChromC7004.4 Software4.4.1 TouchscreenoverviewFig.8:Overviewtouchscreen1 Menuand

BÜCHILabortechnikAG Descriptionoffunction|4PrepChromC700 27/110Icon MenufunctionDialogwindowDescriptionFile OpenData CurrentMethod–ope

4|Descriptionoffunction BÜCHILabortechnikAG28/110 PrepChromC700Icon MenufunctionDialogwindowDescriptionSetup ViewMethod ViewCurrentMeth

BÜCHILabortechnikAG Descriptionoffunction|4PrepChromC700 29/110Icon MenufunctionDialogwindowDescriptionControl Start Startsthemethodman

BÜCHILabortechnikAG TableofcontentsPrepChromC700 iiiTableofcontents1 General...

4|Descriptionoffunction BÜCHILabortechnikAG30/110 PrepChromC700Menu Allowsto:Setup ViewcurrentmethodEditcurrentandsavedmethodSetupbott

BÜCHILabortechnikAG Descriptionoffunction|4PrepChromC700 31/1104.4.5 SynopticFig.10:Changeableparametersduringarun1 Gradient 2 Pumppres

4|Descriptionoffunction BÜCHILabortechnikAG32/110 PrepChromC7004.4.6 StopmodesCorrespondstothewaythesystembehavesattheendofthemet

BÜCHILabortechnikAG Descriptionoffunction|4PrepChromC700 33/1104.4.7 Collection4.4.7.1 CollectallFig.12:Collectall1 Chromatogram/peak 2 Co

4|Descriptionoffunction BÜCHILabortechnikAG34/110 PrepChromC7004.4.7.3 WasteFig.14:Collectiontowaste1 Chromatogram 2 CollectiontimeDuring

BÜCHILabortechnikAG Descriptionoffunction|4PrepChromC700 35/1104.5.2 BackflushvalveThebackflushvalveisusedtoflushthecolumnbackward

4|Descriptionoffunction BÜCHILabortechnikAG36/110 PrepChromC7004.5.3 PurgevalveThepurgevalveisusedtorinsethesolventlinewithoutdisc

BÜCHILabortechnikAG Descriptionoffunction|4PrepChromC700 37/1104.5.4 FumeenclosureThefumeencloseallowstoworkwithoutusingafumehood.

4|Descriptionoffunction BÜCHILabortechnikAG38/110 PrepChromC700

BÜCHILabortechnikAG Installation|5PrepChromC700 39/1105 Installation5.1 Modules,assemblyFig.15:Assemblyorder1 Fractioncollectormodule 2 Pu

Tableofcontents BÜCHILabortechnikAGiv PrepChromC7004.4.4 Racksandglasstubesoverview ...

5|Installation BÜCHILabortechnikAG40/110 PrepChromC700u Positionthecolumnholder(2)ontheleftsideandsecureitwith2hexscrews(1),wash

BÜCHILabortechnikAG Installation|5PrepChromC700 41/1105.2 Tubing,installation5.2.1 6wayelectricalvalve,overviewInjection adapterPre-ColumnC

5|Installation BÜCHILabortechnikAG42/110 PrepChromC700NOTICERiskofpumpdamage.Theport"air"onthe4wayaspirationvalveisfora

BÜCHILabortechnikAG Installation|5PrepChromC700 43/110u Installtheloop(2)betweentheportno2and5.• HAF560Injectionloop,2mL5.2.3 Tubi

5|Installation BÜCHILabortechnikAG44/110 PrepChromC700Followontasksu 5.5.1 Flash/prep column, preparation [} page47]5.2.4 TubingprepHPLCco

BÜCHILabortechnikAG Installation|5PrepChromC700 45/110Followontasksu 5.5.3 Prep HPLC column, installation [} page48]5.3 Electrical,connectio

5|Installation BÜCHILabortechnikAG46/110 PrepChromC7005.3.2 Externaldevices,connectionNOTICERiskofsoftwaredamageduetomalware.Noantivir

BÜCHILabortechnikAG Installation|5PrepChromC700 47/1105.5 Column,preparation5.5.1 Flash/prepcolumn,preparationCAUTIONRiskofchemicalburns

5|Installation BÜCHILabortechnikAG48/110 PrepChromC700u Tightentheknurledknob(1)tosecuretheupperholder.5.5.2 Dryloadcolumn,preparati

BÜCHILabortechnikAG Installation|5PrepChromC700 49/110u MovetheupperholderdownontothecolumnandtightenthePEEKadapter(5).u Tightenthe

BÜCHILabortechnikAG TableofcontentsPrepChromC700 v6.11 Tracing...

5|Installation BÜCHILabortechnikAG50/110 PrepChromC7005.7 Software,setup5.7.1 Systemconfiguration,setupNOTEThecommunicationsetuphastobe

BÜCHILabortechnikAG Installation|5PrepChromC700 51/110u Activate/DeactivateAirPurge(3)ifneeded.u SelectthetabDetector.u Clickintothef

5|Installation BÜCHILabortechnikAG52/110 PrepChromC700u SelectthetabValves.u Clickintothefield(1)tofindtherightCOMport.Allmodules

BÜCHILabortechnikAG Installation|5PrepChromC700 53/110u ChooseGlobalSignalorSeparateSignal.5.7.2 Userprofileu PressSetup>UserProfil

5|Installation BÜCHILabortechnikAG54/110 PrepChromC700Pressure ChangethecolorofPressureplot(=issuedofPressureTransducer)anditscurve

BÜCHILabortechnikAG Installation|5PrepChromC700 55/110DetectorLampCoolingTimeIndicator Adjustthetimeofdisplayofthedialogboxwhichgive

5|Installation BÜCHILabortechnikAG56/110 PrepChromC700Column EnableordisablethemodificationofcolumnsavailableinthedatabaseRacks Enable

BÜCHILabortechnikAG Installation|5PrepChromC700 57/110GeneralPanelWhenEditingaMethod Chooseiftheeditionofamethodcanbeperformeddirec

5|Installation BÜCHILabortechnikAG58/110 PrepChromC700ElutionPanelGradientManagement Choosethewaytomanagethemodificationofgradientsd

BÜCHILabortechnikAG Installation|5PrepChromC700 59/110Curves Showorhidethecurvesobtained/platted(=curvespanel)onthemethodreportPea

Tableofcontents BÜCHILabortechnikAGvi PrepChromC700

5|Installation BÜCHILabortechnikAG60/110 PrepChromC7005.7.3 Bottle,setupu PressSetup>Bottlesonthemainmenu.u Adjustthevalueofeach

BÜCHILabortechnikAG Installation|5PrepChromC700 61/110u PressImportorExport(5)torecoverorsavethecurrentcolumnslist(files*.cart)NOTE

5|Installation BÜCHILabortechnikAG62/110 PrepChromC700Fig.17:Fractioncollectoroffsetadjustment1 OffsetX=1 2 OffsetY=23 SpacingX=3 4 Spac

BÜCHILabortechnikAG Installation|5PrepChromC700 63/1105.7.7 Solvent,setupu PressSetup>Solventsonthemainmenu.u PressAddtodefineall

5|Installation BÜCHILabortechnikAG64/110 PrepChromC7005.7.9 Language,changeu PressView>Languageandselectthedesiredlanguage.• Thesof

BÜCHILabortechnikAG Installation|5PrepChromC700 65/1105.7.11 Detectionstep,activation/deactivationu PressSetup>UserProfileonthemain

5|Installation BÜCHILabortechnikAG66/110 PrepChromC7005.7.13 Automaticgradientoptimization,activation/deactivationu PressSetup>UserPro

BÜCHILabortechnikAG Installation|5PrepChromC700 67/110u SelectthevalvetypeACTV(1),searchfortherightCOMport(2),determinetheconfig

5|Installation BÜCHILabortechnikAG68/110 PrepChromC700NoteColumn1orcolumn2canbealsoselectedinthesynopticbyclickingonthecolumn1o

BÜCHILabortechnikAG Installation|5PrepChromC700 69/110u Determinethedirectionoftheflowthroughthecolumn(Dir=Direct,Rev=Reverse)inth

BÜCHILabortechnikAG General|1PrepChromC700 7/1101 General1.1 AboutthismanualReadthismanualcarefullybeforeinstallingandrunningthedevic

5|Installation BÜCHILabortechnikAG70/110 PrepChromC7005.8 Systemfixation(earthquake)NOTICERiskofdevicedamagefromearthquakes.Anunsecured

BÜCHILabortechnikAG Operation|6PrepChromC700 71/1106 OperationCAUTIONRiskoffireduetoleakages.Solventcanescapethesystemincaseofale

6|Operation BÜCHILabortechnikAG72/110 PrepChromC7006.3 CreateaquickmanualmethodFig.18:Overviewtouchscreen1 Menuandtoolbar 2 Rackand

BÜCHILabortechnikAG Operation|6PrepChromC700 73/110u Selectthedetectorchannel(s)andwavelength.u Selecttherack.

6|Operation BÜCHILabortechnikAG74/110 PrepChromC700u Selectglasstubevolume.u Selecttheequilibrationandadjustthedurationtimeintheupp

BÜCHILabortechnikAG Operation|6PrepChromC700 75/110u PressStart.6.4 Createanewmethodu Pressthefilemanagementbuttononthemainscreen.u

6|Operation BÜCHILabortechnikAG76/110 PrepChromC700u SelectthetabSettings.u Selectacolumnfromthedropdownmenu(1).u Selecttheequilibr

BÜCHILabortechnikAG Operation|6PrepChromC700 77/110u SelectthetabElution.u Presstheicon(1)toswitchbetweenthegraphicalandclassicalt

6|Operation BÜCHILabortechnikAG78/110 PrepChromC700• StepgradientmodeNoteThefirststepisusedasastartpointonlyandcorrespondstothec

BÜCHILabortechnikAG Operation|6PrepChromC700 79/110u Selectthedesiredsignal(1)forthechannel1–4orusethescanmode.u Adjustthewavelen

1|General BÜCHILabortechnikAG8/110 PrepChromC700NoteNOTEParagraphsstartingwithNOTEtransporthelpfulinformationforworkingwiththedevice/s

6|Operation BÜCHILabortechnikAG80/110 PrepChromC700NOTICERiskofspilledliquidsduetowrongglasstubes.Liquidisspilledbythefractioncol

BÜCHILabortechnikAG Operation|6PrepChromC700 81/1106.5 Equilibrationandinjectionmodesu Chooseanequilibrationmodetoconditionthecolumn

6|Operation BÜCHILabortechnikAG82/110 PrepChromC700u Selecttheinjectionmode(equilibrationandvalveinjection).u Afterstartofthemethodt

BÜCHILabortechnikAG Operation|6PrepChromC700 83/110u Selectthesolventnames(1)fortheLowElutionForce(X)andforHighElutionForce(Y).u

6|Operation BÜCHILabortechnikAG84/110 PrepChromC700u SelectLiquidInjectionorDryLoading(1)dependingoftheapplication.• IfLiquidInjecti

BÜCHILabortechnikAG Operation|6PrepChromC700 85/1106.8 PurgeNOTEPurgethesystembeforethefirstuseorwhenthesolventinthebottlehasbeen

6|Operation BÜCHILabortechnikAG86/110 PrepChromC7006.10 DatastorageTheresultsarestoredin4filesofwhichtherootofthenameisidentical

BÜCHILabortechnikAG Maintenance|7PrepChromC700 87/1107 MaintenanceWARNINGRiskofhandinjury.Movingpartse.g.pulleyorabeltcancauseserio

7|Maintenance BÜCHILabortechnikAG88/110 PrepChromC700u Purgethesystemwithasuitablesolventoralcohol(1propanolor2propanol)duringapp

BÜCHILabortechnikAG Maintenance|7PrepChromC700 89/110u Holdtheflowcell(2)withonehandwhileliftingthelever(1)furtherup.u Removethe

BÜCHILabortechnikAG General|1PrepChromC700 9/110Fig.1:Typeplate(example)1 Companyname 2 Devicename3 Serialnumber 4 Inputvoltagerange5 M

7|Maintenance BÜCHILabortechnikAG90/110 PrepChromC7007.2.4 Pistoncleaningdisc,cleaningNOTICERiskofelectricaldamage.Connectingthepiston

BÜCHILabortechnikAG Maintenance|7PrepChromC700 91/110u Remove2countersunkscrewsontopoftheUVDetectormodule.u Remove4hexscrews(1)an

7|Maintenance BÜCHILabortechnikAG92/110 PrepChromC700NOTICERiskofflowcellwindowdamage.Touchingtheflowcellwindowswithbarehandscand

BÜCHILabortechnikAG Maintenance|7PrepChromC700 93/110u 7.3.1 UV Detector cover, removal [} page90].Tasksu LoosentheTORXscrew(1).• TORXscre

7|Maintenance BÜCHILabortechnikAG94/110 PrepChromC700Tasksu Installthelamp(1).NOTEThelamphasapredefinedinstallationposition.u Tightent

BÜCHILabortechnikAG Maintenance|7PrepChromC700 95/110u Airpurgethesystem.• 6.9 Air purge [} page85]Tasksu Opentheservicedoorofthepump

7|Maintenance BÜCHILabortechnikAG96/110 PrepChromC700Fig.19:Checkvalveinstallationposition,pumpinletFig.20:Checkvalveinstallationpos

BÜCHILabortechnikAG Troubleshooting|8PrepChromC700 97/1108 Troubleshooting8.1 CustomerserviceOnlyauthorizedservicepersonnelareallowedtop

8|Troubleshooting BÜCHILabortechnikAG98/110 PrepChromC700

BÜCHILabortechnikAG Takingoutofoperation|9PrepChromC700 99/1109 Takingoutofoperation9.1 TransportPreliminarytasksu Preparetheoriginalp