Buchi ELS Detector C-650 Uživatelský manuál stáhnout pdf (Strana 25)

C-650 Operation Manual, Version B

5 Putting into operation

5.6 Installing the ﬂow splitter

5.6.1 Connecting the ﬂow splitter

The ﬂow splitter comes as complete kit (article number 11059007) and has to be mounted inside

the detector as shown below:

Fig 5.12: Detection chamber/Flow splitter

a Fastening screw e Inline ﬁlter

b Flow adjustment knob f Flow from column outlet

c Union ﬁtting to connect the splitter to

the nebulizer

g Flow to ELSD nebulizer

h Restriction capillary ID 0.10mm

d Flow to fraction collector

1. Fasten the splitter with the fastening screwa to the left side of the ELSD.

2. Connect the following tubings to the splitter kit:

• Outletf from the column

• Connection tubingd to the fraction collector

NOTE

Do not connect the splitter to the ELSD nebulizer before having adjusted the ﬂow rate to

1mL/min.

Recommendation:

For preparative chromatography applications connect the column to the splitter after the condi-

tioning step.

5.6.2 Adjusting the ﬂow rate of the ELSD splitter

1. Remove the protecting sticker from the flow adjustment knob and keep the predefined

adjustment.

2. Start the pumping system with the appropriate eluent for your application.

3. Check the flow rate. If the flow rate is not adjusted at 1mL/min, adjust the flow rate with

the flow adjustment knob until the delivered flow rate represents about 1drop/second,

which is about 1mL/min (±10%). Measure the flow rate using a graduated cylinder

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Buchi ELS Detector C-650 Uživatelský manuál Strana 25